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Registros recuperados: 35
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Estimation du polymorphisme enzymatique d'une population de Penaeus jap nicus maintenue en élevage depuis quatre générations ArchiMer
Laubier, A; Pasteur, N; Moriyasu, M.
The polymorphism of 37 enzyme-encoding genes was investigated in a Penaeus japonicus population born the same night from a stock maintained artificially four generations. Five loci were found to be polymorphie (Lap-4, Es-3, Es-6, Es-8 and Mpi-2), and genotype frequencies at four of these loci indicate: a) that the shrimps studied come from the same two parents and b) that Mpi-2 and Es-3 genes are autosomal. The rate of polymorphism observed in this Penaeus japonicus population is similar to that described in natural populations of other Peneidae species, despite the probable occurrence of inbreeding.
Tipo: Text Palavras-chave: Penaeus japonicus; Aquaculture; Génétique; Allozym; Penaeus japonicus; Aquaculture; Genetics; Allozymes.
Ano: 1984 URL: http://archimer.ifremer.fr/doc/00113/22443/20130.pdf
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Morphological and allozyme studies of midwife toads (genus Alytes), including the description of two new taxa from Spain Naturalis
Arntzen, J.W.; García-París, M..
Allozyme variation in 31 to 50 presumptive loci of 12 populations of European midwife toads of the genus Alytes show appreciable genetic divergences (DiNe from 0.29 to 0.72) among four groups. These groups correspond to A. cisternasii Boscá, 1879, A. obstetricans (Laurenti, 1768), A. muletensis (Sanchiz & Adrover, 1979), and a new species from the Betic mountains described here as A. dickhilleni n. sp. Smaller divergences among geographic groups of populations of A. obstetricans (up to DNei = 0.17) support the existence of three geographic units in Europe corresponding to the previously recognized subspecies A. o. obstetricans and A. o. boscai Lataste, 1879, plus a third one described here under the designation of A. o. almogavarii n. ssp. The analysis...
Tipo: Article / Letter to the editor Palavras-chave: Allozymes; Alytes; Midwife toads; Morphometry; Spain; Taxonomy.
Ano: 1995 URL: http://www.repository.naturalis.nl/record/504393
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Phylogeny and biogeography of midwife toads (Alytes, Discoglossidae): a rebuttal Naturalis
Arntzen, J.W.; García-París, M..
Three competing phylogenetic hypotheses for the genus Alytes (midwife toads) are evaluated. Based on quantitative coding of protein characters the most parsimonious solution shows a sister taxon relationship for Alytes dickhilleni and A. muletensis. The alternatives in which A. obstetricans has its sister group in either A. dickhilleni or A. muletensis lack support. Using calibrations derived from protein evolutionary rates, the vicariant events giving rise to A. obstetricans and the lineage leading to the A. muletensis and A. dickhilleni clade and the subsequent splitting between A. muletensis and A. dickhilleni cannot be placed much earlier than the Miocene-Pliocene boundary. Biogeographical scenarios invoking an earlier time of divergence should be...
Tipo: Article / Letter to the editor Palavras-chave: Allozymes; Alytes; Biogeography; Midwife toads; Phylogenetics; Spain; Taxonomy; Vicariance.
Ano: 1997 URL: http://www.repository.naturalis.nl/record/504096
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International study on <i>Artemia</i> : LX. Allozyme data suggest that a new <i>Artemia</i> population in southern Chile (50° 29' S; 73° 45' W) is <i>A. persimilis</i> OMA
Gajardo, G.; Mercado, C.; Beardmore, J.A.; Sorgeloos, P..
An <i>Artemia</i> population from a site recently described in Chile and located in a semi-arid and humid hydrographical zone (Torres del Paine National Park), was electrophoretically characterized and cross-bred (followed through <i>F</i><sub>3</sub>) to reference samples of <i>A. franciscana</i> (San Francisco Bay, California, USA) and <i>A. persimilis</i> (Buenos Aires, Argentina), which are the species most likely to be found in Chile. The population exhibited high levels of genetic variability as judged by the percentage of polymorphic loci (<i>P</i>=53.3), mean number of alleles per locus (<i>n</i>=2.2) and mean heterozygosity (<i>H</i>=0.176). Based on...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Allopatric populations; Allozymes; Genetic isolation; Hybridization; Population genetics; Chile.
Ano: 1999 URL: http://www.vliz.be/nl/open-marien-archief?module=ref&refid=4016
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Spatial genetic structure within natural populations of Machaerium villosum Vog. (Leguminosae) at Moji-Guaçu region, SP, Brazil Rev. Bras. Bot.
GIUDICE NETO,JOÃO DEL; KAGEYAMA,PAULO YOSHIO.
A spatial autocorrelation study of enzyme loci detected by starch gel electrophoresis was performed to verify the occurrence of spatial genetic structure within two natural populations of Machaerium villosum Vog. The sampled populations were termed "Antropic Model (MA)" and "Natural Model (MN)" and they are situated in Campininha Farm areas, at Moji-Guaçu municipality, 22°10'43''-22°18'19'' S and 47°8'5"-47°11'34" W, in the state of São Paulo. Ten polymorphic loci in the MA population and nine polymorphic loci in the MN population were assessed by Moran's I autocorrelation statistic. No spatial autocorrelation was detected among individuals within sampled populations. Results are in line with other studies in woody species from tropical rain forest.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Machaerium villosum; Allozymes; Spatial autocorrelation.
Ano: 2000 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-84042000000200010
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Genetic and ecological differentiation between the butterfly sisterspecies Colias alfacariensis and Colias hyale Naturalis
Cleary, Daniel F.R.; Descimon, Henri; Menken, Steph B.J..
Clear habitat separation between the sister species Colias alfacariensis and C. hyale is shown when occurring sympatrically. Colias hyale is found more often in moist cultivated pastures while Colias alfacariensis is more abundant in dry uncultivated habitat. Out of a total of 16 loci, no diagnostic loci were found between C. alfacariensis and C. hyale, and both species shared most major polymorphisms. Exceptions were the marked differences in allele frequencies at the HK locus and only C, hyale, but not C. alfacariensis was further invariable at the GOT2 locus, which is usually highly polymorphic in the Pieridae. Colias hyale has a significantly lower level of heterozygosity than its sister species C. alfacariensis. In Colias alfacariensis heterozygosity...
Tipo: Article / Letter to the editor Palavras-chave: Lepidoptera; Pieridae; Allozymes; Population structure; Gene flow.
Ano: 2002 URL: http://www.repository.naturalis.nl/record/534385
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Comparative genetic structure in pines: evolutionary and conservation consequences RChHN
DELGADO,PATRICIA; CUENCA,ARGELIA; ESCALANTE,ANA E.; MOLINA-FREANER,FRANCISCO; PIÑERO,DANIEL.
Pines have been the focus of several studies that estimate population genetic parameters using both allozymes and chloroplast single sequence repeats (SSRs). Also, the genus has also been recently studied using molecular systematics so that we now have a more clear understanding of their evolutionary history. With this background we studied comparatively the genetic structure in pines. Expected heterozygosity is particularly constant with a 99 % confidence interval between 0.19 and 0.23 in species that have been studied until now using allozymes. There is a significant proportion of species (9/41) that show high population differentiation estimates (Fst = or larger than 0.15) and five of these have large and wingless seeds probably associated with low...
Tipo: Journal article Palavras-chave: Pinus; Genetic structure; Outcrossing rates; Microsatellites; Allozymes.
Ano: 2002 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-078X2002000100004
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Genetic structure of honeybee populations from southern Brazil and Uruguay Genet. Mol. Biol.
Diniz,Nilza Maria; Soares,Ademilson Espencer Egea; Sheppard,Walter Steve; Del Lama,Marco Antonio.
Apis mellifera scutellata was introduced to Brazil in 1956 and Africanized honeybee populations have now spread from Argentina to the southwestern United States. Temperate climatic restrictions seem to be a natural limit to Africanized honeybee expansion around parallels 35° to 40° SL. We used allozyme loci (Mdh-1 and Hk-1) and mtDNA haplotypes to characterize honeybee populations in southern Brazil and Uruguay and define a possible transition area between Africanized and European bees. Samples of 194 bee colonies were collected from ten localities between 30°-35° SL and 52°-59° WL. The mtDNA restriction patterns of these colonies were obtained through digestion of the mitochondrial genome by Eco RI, or by digestion by Bgl II and Xba I of the cytochrome B...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Africanized honeybees; Transition area; South America; MtDNA; Allozymes; Population genetics.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000100008
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Genetic structure of bisexual and parthenogenetic populations of Artemia from Italian brackish-hypersaline waters ArchiMer
Nascetti, G; Bondanelli, P; Aldinucci, A; Cimmaruta, R.
Eight populations of Artemia displaying different reproduction modalities (amphigonic vs. parthenogenetic) and ploidies (diploid vs. polyploid) have been collected from Italian salt-works or brackish coastal lagoons, and their genetic structure has been investigated using allozyme electrophoresis. The results obtained on genetic differentiation showed that the Tyrrhenian bisexual samples (A. salina) are genetically homogeneous even between populations located in relatively faraway sites (Sardinia-Latium), having a moderate gene flow assisted by migratory waterfowl. The parthenogenetic samples (A. parthenogenetica) are genetically well differentiated both from the bisexual ones and among them, even when characterised by the same ploidy. Each sample presents...
Tipo: Text Palavras-chave: Artemia; Allozymes; Reproduction amphigonique; Parthénogenèse; Hétérozygotie; Artemia; Allozymes; Amphigony; Parthenogenesis; Heterozygosity.
Ano: 2003 URL: http://archimer.ifremer.fr/doc/00322/43289/43024.pdf
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Genetic diversity and environmental stress in Italian populations of the cyprinodont fish Aphanius fasciatus ArchiMer
Cimmaruta, R; Scialanca, F; Luccioli, F; Nascetti, G.
Allozyme electrophoresis has been used to analyse the genetic structure of ten populations of the killifish, Aphanius fasciatus, sampled from Italian coastal brackish lagoons or salterns. The goal of the study was to compare the genetic structure of samples obtained from habitats characterised by environmental stress of different kinds, with the aim of gaining a better understanding of the relationships between the genetic variation of natural populations and environmental disturbance, either natural or man-made. Therefore, the sampling scheme has been planned to provide samples from both brackish habitats, characterised by natural stress with seasonal marked fluctuations of chemical and physical parameters, and from coastal lagoons suffering from a high...
Tipo: Text Palavras-chave: Aphanius fasciatus; Hétérozygotie; Allozymes; Eaux saumâtres; Stress du milieu; Aphanius fasciatus; Heterozygosity; Allozymes; Brackish waters; Environmental stress.
Ano: 2003 URL: http://archimer.ifremer.fr/doc/00322/43290/43025.pdf
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Populational genetic structure and sociogenetic structure of cocoon masses of Digelasinus diversipes (Kirby, 1882) (Hymenoptera: Symphyta: Argidae) Genet. Mol. Biol.
Boraschi,Daniele; Del Lama,Marco Antonio.
Gene variation and population genetic structure of the Neotropical sawfly Digelasinus diversipes were measured by allozyme analyses using starch gel electrophoresis. Cocoon masses were collected in Eugenia glazioviana (Myrtaceae) stems, in two areas of the "Estação Ecológica Jataí" (Luiz Antônio, SP, Brazil - 21°25' S, 47°50' W), in 2000 and 2001. The average heterozygosity observed in this species (Hobs = 0.094 ± 0.025) was not significantly different from other Symphyta groups; it was, however, significantly higher than in other Hymenoptera populations. No significant levels of inbreeding were found (F IS = 0.062; chi2 = 29.9; p > 0.05), but the population was subdivided (F ST = 0.070; chi2 = 458.9; p < 0.05), suggesting the absence of a...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Genetic variability; Population genetics; Digelasinus diversipes; Neotropical sawfly; Allozymes.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000300013
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Genetic structure of natural populations of Cryptocarya moschata Nees (Lauraceae) from southeastern Brazilian Atlantic rain forest Biota Neotropica
Moraes,Pedro Luís Rodrigues de; Derbyshire,Maria Teresa Vitral de Carvalho.
This study was accomplished on a more comprehensive basis to evaluate previous questions that were raised from a preliminary article about the genetic structure of Cryptocarya moschata populations. Thus, through the analysis of 40 polymorphic allozyme loci, allele frequencies were estimated from 335 individuals of 11 natural populations of C. moschata from six hydrographic basins of São Paulo state and Serra da Estrela, Rio de Janeiro, Brazil. Estimates of Wright's F statistics were done through the analysis of variance, presenting average values of <img border=0 width=32 height=32 id="_x0000_i1026" src="../../../../../../img/revistas/bn/v4n1/img/a04car(f).jpg" align=absmiddle > or = 0.352, <img border=0 width=32 height=32 id="_x0000_i1027"...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Allozymes; Lauraceae; Genetic structure; Neotropics; Cryptocarya moschata; Atlantic rain forest; Brazil.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1676-06032004000100004
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Genetic variability in Astyanax altiparanae Garutti &amp; Britski, 2000 (Teleostei, Characidae) from the Upper Paraná River basin, Brazil Genet. Mol. Biol.
Peres,Maria Dolores; Vasconcelos,Marcio dos Santos; Renesto,Erasmo.
Allozyme data was used to assess the genetic diversity Astyanax altiparanae populations from the floodplain of the Upper Paraná River (PR). Specimens were collected in the southern Brazilian state of Paraná from PR in Porto Rico municipality and Ribeirão Ficha (RF) in Ubiratã municipality. The authors used 15% (w/v) corn starch gel electrophoresis to identify 21 putative loci for 13 enzymatic systems: Aspartate aminotransferase, 2.6.1.1 (AAT), Acid phosphatase, 3.1.3.2 (ACP), Esterase, 3.1.1.1 (EST), Glycerol-3-phosphate dehydrogenase, 1.1.1.8 (G3PDH), Glucose-6-phosphate dehydrogenase, 1.1.1.49 (G6PDH), Glucose-6-phosphate isomerase, 5.3.1.9 (GPI), Iditol dehydrogenase, 1.1.1.14 (IDDH), Isocitrate dehydrogenase - NADP+, 1.1.1.42 (IDH), L-Lactate...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Allozymes; Astyanax; Fishes; Genetic variability; Paraná River floodplain.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000500011
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Population genetic structure of Brazilian shrimp species (Farfantepenaeus sp., F. brasiliensis, F. paulensis and Litopenaeus schmitti: Decapoda: Penaeidae) Genet. Mol. Biol.
Gusmão,Jaqueline; Lazoski,Cristiano; Solé-Cava,Antonio M..
Penaeid shrimps are important resources for worldwide fisheries and aquaculture. In the Southwest Atlantic, Farfantepenaeus brasiliensis, F. paulensis, F. subtilis, Farfantepenaeus sp. and Litopenaeus schmitti are among the most important commercially exploited species. Despite their high commercial value, there is little information available on the different aspects of their biology or genetics and almost no data on their stock structure. We used allozymes to estimate variability levels and population genetic structure of F. brasiliensis, F. paulensis, L. schmitti and the recently detected species Farfantepenaeus sp. along as much as 4,000 km of Brazilian coastline. No population heterogeneity was detected in F. brasiliensis or L. schmitti along the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Farfantepenaeus; Litopenaeus; Allozymes.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000100029
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Genetic variability of Hypostomus (Teleostei, Loricariidae) from the Ribeirão Maringá, a stream of the upper Rio Paraná basin, Brazil Genet. Mol. Biol.
Paiva,Suzana de; Renesto,Erasmo; Zawadzki,Cláudio Henrique.
Hypostomus strigaticeps and two morphotypes of Hypostomus were collected from Ribeirão Maringá, a small tributary of the Rio Pirapó, an effluent of the upper Rio Paraná. The three populations were analyzed by allozyme electrophoresis that allowed the scoring of 25 loci from 14 enzyme systems. Heterozygosity values (He) were 0.028 in H. strigaticeps, 0.027 in Hypostomus sp. 1 and zero in Hypostomus sp. 2. Several diagnostic loci and fixed differences were observed for each population at loci Acp-A, Gcdh-A and Mdhp-A. Thus, all populations were genetically distinct, although there were many common alleles. The unbiased genetic identities of Nei (I) were estimated as 0.780 for Hypostomus sp. 1 and H. strigaticeps, 0.357 for H. strigaticeps and Hypostomus sp....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Allozymes; Heterozygosity; Hypostomus; Loricariidae; Systematic.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000300005
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Sharp genetic break between Atlantic and English Channel populations of the polychaete Pectinaria koreni, along the North coast of France ArchiMer
Jolly, Mt; Jollivet, D; Gentil, F; Thiebaut, E; Viard, F.
This study uses enzymatic and mitochondrial genes to infer the relative importance of historical processes and contemporary hydrodynamic features on the observed patterns of genetic structure in subdivided populations of Pectinaria koreni (Polychaeta: Pectinariidae) along the coasts of Brittany and the English Channel. Nucleotide sequence variation of a 603-bp fragment of the mtDNA cytochrome oxidase subunit I gene revealed a surprisingly deep phylogeographic break of about 16% divergence separating the Brittany and Channel populations, which coincides with a biogeographic boundary along the western coast of Brittany. Deep sequence divergence with fixed haplotype differences and the inversion of allele frequencies at two enzyme loci suggests the occurrence...
Tipo: Text Palavras-chave: Phylogeography; Allozymes; MtDNA; Parapatric boundary; Cryptic species.
Ano: 2005 URL: https://archimer.ifremer.fr/doc/00301/41250/40433.pdf
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Molecular research and the control of Chagas disease vectors Anais da ABC (AABC)
Abad-Franch,Fernando; Monteiro,Fernando A..
Chagas disease control initiatives are yielding promising results. Molecular research has helped successful programs by identifying and characterizing introduced vector populations and by defining intervention targets accurately. However, researchers and health officials are facing new challenges throughout Latin America. Native vectors persistently reinfest insecticide-treated households, and sylvatic triatomines maintain disease transmission in humid forest regions (including Amazonia) without colonizing human dwellings. In these scenarios, fine-scale vector studies are essential to define epidemiological risk patterns and clarify the involvement of little-known triatomine taxa in disease transmission. These eco-epidemiological investigations, as well as...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Molecular systematics; DNA; Allozymes; Triatominae; Chagas disease.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652005000300007
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Variance of reproductive success in the European flat oyster Ostrea edulis assessed by microsatellite-based parentage analyses in a natural population and in hatchery ArchiMer
Lallias, Delphine; Taris, Nicolas; Boudry, Pierre; Bonhomme, Francois; Lapegue, Sylvie.
he European flat oyster (Ostrea edulis L.) is a marine bivalve whose natural geographical distribution ranges along the European Atlantic coast from Norway to Morocco, in addition to the Mediterranean and Black Sea. Previous studies of allozymes, microsat-ellites and mitochondrial differentiation over the whole range concluded that a significant divergence existed between Mediterranean and Atlantic populations, together with an isolation-by-distance pattern. However, the average mitochondrial haplotypic diversity displayed a high among populations variance, reflecting smaller effective population size in some locations. Additionally, a ten-fold quantitative difference was observed in the same study in Fst between the mitochondrial and the nuclear genomes,...
Tipo: Text Palavras-chave: Genotypes; Genetic diversity; Breeding success; Allozymes; Population genetics; Parentage analyses; Natural population; Ostrea edulis; Oyster.
Ano: 2006 URL: http://archimer.ifremer.fr/doc/2006/acte-3334.pdf
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Phenotypic plasticity revealed by molecular studies on reef corals of Fungia (Cycloseris) spp. (Scleractinia: Fungiidae) near river outlets Naturalis
Gittenberger, A.; Hoeksema, B.W..
On a patch reef off Makassar, Sulawesi, Indonesia, corals identified as Fungia (Cycloseris) costulata, Fungia (Cycloseris) tenuis and Fungia (Cycloseris) cf costulata were collected down to a maximum depth of 10 m. The corals lived sympatrically. Mushroom coral clones resulting from fragmentation can be recognized by their equal coloration and close proximity. Therefore, to ensure that no clones were collected, corals of dissimilar colors were selected at a mutual distance of 5 m. The corals were kept alive in two 30 liter sea-water aquariums with an air-pump. They were photographed in detail. Using allozyme electrophoresis in a laboratory close to the field area, it was tested whether the separate coral morphs should be considered three species....
Tipo: Article / Letter to the editor Palavras-chave: Eco-phenotype; Plasticity; Allozymes; River outlets; Mushroom corals; Fungiidae; Cycloseris; Indonesia; 42.79.
Ano: 2006 URL: http://www.repository.naturalis.nl/record/214568
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Allozyme variation and sociogenetic structure of Polistes satan Bequaert 1940 colonies (Hymenoptera, Vespidae) Genet. Mol. Biol.
Gaspar,Jane Cristina Wehren; López-Uribe,Margarita María; Del Lama,Marco Antônio.
We collected 40 colonies of the wasp Polistes satan in November 1993 (Population A = 23 colonies) and October 1995 (Population B = 17 colonies) from the town of Delfinopólis in the Brazilian state of Minas Gerais. Pupae from these nests were used to assess allozyme variation and genetic relatedness among nestmates. Of the 24 gene loci sampled six showed allozyme polymorphism (Est-2, Pgm-1, 6Pgd, Hk-1, Idh and Pep-A) and all loci except Idh were at Hardy-Weinberg equilibrium. Populations A and B showed distinct intra-loci heterozygosities, but similar average heterozygosity over all loci. Significant inter-population differentiation (Fst = 0.051; p < 0.01) was detected, probably due to genetic drift driven by bottlenecking that occurred due to the first...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Allozymes; Gene variation; Polistes satan; Relatedness; Wasps.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000300028
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